Anti-MDM2 (Ab-166) Antibody

MDM2 (Mouse Double Minute 2 homolog) is an E3 ubiquitin-protein ligase and the principal negative regulator of the p53 tumor suppressor pathway, targeting p53 for ubiquitin-mediated proteasomal degradation. This rabbit polyclonal antibody from Antibodies.com is raised against a synthetic peptide corresponding to a region surrounding residue 166 of human MDM2. The unconjugated reagent is supplied at 100 µl and is validated for Western blot, immunohistochemistry, and immunofluorescence on human samples. Investigators working in oncology, DNA damage response, and cell cycle research rely on this reagent to monitor endogenous MDM2 levels in tumor tissue and cell lines, follow pathway activation after genotoxic stress, and visualize subcellular localization of the protein. Affinity purification from rabbit serum supports sensitive detection across a range of lysate inputs and staining protocols.