Anti-MDH1 Antibody

Malate dehydrogenase 1 (MDH1) is a cytoplasmic NAD-dependent enzyme that catalyzes the reversible oxidation of malate to oxaloacetate, linking the citric acid cycle, gluconeogenesis, and the malate-aspartate shuttle that moves reducing equivalents between cytosolic and mitochondrial pools. This unconjugated Rabbit polyclonal primary antibody from Antibodies.com was raised against human MDH1 and validated for Western blot detection across human, mouse, and rat samples.

The polyclonal format recognizes multiple epitopes on the MDH1 protein, supporting robust signal generation on western blot membranes and reliable detection of endogenous MDH1 in cell lysates and tissue homogenates. Cross-species reactivity makes the reagent useful for comparative metabolic studies, including investigations of cancer metabolism, liver biology, and mitochondrial dysfunction, where MDH1 expression shifts under varying physiological and pathological conditions.

Each vial contains 100 µl of antibody supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, allowing convenient storage at -20°C and resistance to repeated freeze-thaw cycles. The unconjugated format provides downstream flexibility with HRP, fluorescent, or biotinylated secondary reagents for diverse detection strategies. Host species is Rabbit; clonality is polyclonal; the target is human MDH1 with confirmed reactivity in mouse and rat samples.