Anti-MCM5 Antibody

The Antibodies.com rabbit polyclonal reagent targets MCM5 (Minichromosome Maintenance Complex Component 5), an essential subunit of the MCM2-7 replicative helicase complex that unwinds parental DNA ahead of the replication fork. The unconjugated antibody is supplied as a 100 µl liquid aliquot at 1 mg/ml in PBS buffer containing 0.1% sodium azide and 50% glycerol as a cryoprotectant for long-term storage. Validation has been performed for Western blot, immunohistochemistry, and immunofluorescence workflows, with demonstrated reactivity against human, mouse, and rat samples.

Because MCM5 expression is tightly coupled to entry into S-phase, the antibody is frequently used as a proliferation marker in tumor biology, regenerative studies, and stem cell research. Investigators apply it to chart DNA replication licensing, identify actively dividing populations in fixed tissue sections, and probe genome stability pathways in cell-based assays. Store the vial at -20 °C in working aliquots to limit freeze-thaw cycles, and protect from light when planning downstream fluorescent immunolabeling experiments.