Anti-MCM3 Antibody

Recognizing human MCM3 (Minichromosome Maintenance 3), this unconjugated rabbit polyclonal antibody binds a core subunit of the MCM2-7 replicative helicase complex, the ring-shaped motor that unwinds parental DNA ahead of the replication fork during S phase. Supplied by Antibodies.com as a 100 µl vial, the reagent is validated for western blot detection of the endogenous protein in human samples and is also compatible with ELISA-based workflows.

MCM3 expression tracks proliferative status, which is why the antibody is widely applied to studies of cell cycle entry, replication licensing, and oncogenic transformation. Researchers use it to characterize replication stress responses, monitor tumor cell growth kinetics, and profile dividing populations in tissue sections where proliferative index matters. For western blot work, aliquot the antibody upon arrival and store at -20 degrees C in working volumes; limiting freeze-thaw cycles preserves binding activity over many experiments.

The polyclonal format offers recognition of multiple epitopes across MCM3, which is useful when probing for full-length protein as well as processed or truncated forms that can arise under different cellular conditions. Reactivity is confirmed against the human target, and the unconjugated preparation leaves the antibody free for downstream labeling with HRP, biotin, or fluorophore conjugates chosen by the user.