Anti-MCM3 Antibody

MCM3 (Minichromosome Maintenance Complex Component 3) is a subunit of the MCM2-7 hexameric helicase that unwinds parental DNA at replication origins during S phase, and this unconjugated rabbit polyclonal reagent supplied by Antibodies.com in a 100 µl vial is used to detect the protein in actively proliferating cells. Researchers apply the antibody in western blot, immunohistochemistry, and immunofluorescence workflows to study DNA replication licensing, origin firing, and proliferation status in tissue sections and cell lysates. Because MCM3 expression rises in cells committed to division and falls in quiescent or senescent populations, the reagent is frequently used to mark replicating tumor cells, monitor cell-cycle re-entry after stress, and compare S-phase engagement across normal and diseased tissue. The antibody has been validated on human, mouse, and rat samples, supporting translational studies that compare replication burden or checkpoint activation across species. Aliquot on first use and store at -20°C to preserve activity across multiple freeze-thaw cycles, avoiding repeated warming of the stock vial.