Anti-MCM2 Antibody

The antibody targets MCM2 (Mini-Chromosome Maintenance protein 2), a core subunit of the MCM2-7 replicative helicase complex that licenses replication origins and unwinds parental DNA during S phase. Raised in rabbit and supplied by Antibodies.com as a polyclonal, unconjugated reagent in 100 µl format, this preparation is validated for Western Blot (WB) and Immunohistochemistry (IHC) workflows, with reactivity confirmed against human, mouse, and rat samples.

Because MCM2 expression marks cells that have exited quiescence and entered the proliferation cycle, it is widely used as a proliferation indicator in tumor biology, often alongside Ki-67, particularly when assessing formalin-fixed paraffin-embedded (FFPE) tissue sections. Researchers studying replication stress, cell cycle re-entry, and tumor growth fraction in cervical, breast, colorectal, and brain tissues routinely employ MCM2 immunostaining.

The unconjugated format offers flexibility for use with HRP- or fluorophore-conjugated secondary reagents, supporting both chromogenic and immunofluorescence detection. Cross-species reactivity against human, mouse, and rat enables translational work spanning cell line panels, rodent disease models, and archived clinical specimens.