Anti-MCAM Antibody

This Rabbit polyclonal IgG antibody targets MCAM (melanoma cell adhesion molecule), a transmembrane glycoprotein also designated CD146 that is widely used as a research marker for endothelial cells, mesenchymal stem cells, pericytes, and several tumor cell populations. Raised in Rabbit and supplied unconjugated in a 200 µL vial by Antibodies.com, the reagent is validated for Western Blot applications and has been tested for reactivity with human, mouse, and rat samples, making it useful for comparative studies across these species in vascular biology, stem cell characterization, and oncology workflows. The polyclonal format offers recognition of multiple epitopes on the MCAM extracellular and intracellular domains, which is advantageous when probing denatured targets on western blots. For handling, aliquot the antibody upon first use to limit freeze-thaw cycles and preserve IgG binding activity. As an unconjugated primary, it can be paired with HRP- or fluorophore-conjugated secondary antibodies chosen by the user, supporting chemiluminescent western blot imaging and downstream quantification of MCAM expression levels in cell lysates and tissue homogenates. Researchers investigating angiogenesis, tumor metastasis, and the mesenchymal stem cell compartment use this reagent to confirm MCAM-positive populations alongside other lineage markers.