Anti-MCAM Antibody

This polyclonal antibody from Antibodies.com targets MCAM (Melanoma Cell Adhesion Molecule), a transmembrane glycoprotein also designated CD146 that is expressed on vascular endothelium, pericytes, activated lymphocytes, and a subpopulation of mesenchymal stem cells. Raised in rabbit and purified as an unconjugated IgG, the reagent is supplied at 100 µl per vial and is validated for Western Blot detection across human, mouse, and rat samples.

MCAM/CD146 is a routinely cited marker in studies of angiogenesis, tumor neovascularization, and metastasis, where investigators use anti-MCAM antibodies to label endothelial lineage cells, isolate pericyte fractions, and characterize circulating tumor cell populations. Western Blot applications resolve MCAM at its expected high-molecular-weight band, making this antibody useful for confirming CD146 expression in endothelial cell lysates, mesenchymal stem cell preparations, and melanoma-derived lines.

Recommended storage is at -20 °C in working aliquots to limit freeze-thaw cycles; brief refrigeration at 4 °C is suitable for short-term use. The unconjugated format allows flexible secondary antibody selection for chemiluminescent or fluorescent Western Blot workflows. Researchers studying tumor microenvironment, vascular biology, and stem cell surface marker panels commonly include this MCAM reagent in their primary antibody panels.