Anti-MBP Tag Antibody

From Antibodies.com, this unconjugated Mouse polyclonal reagent detects the Maltose Binding Protein (MBP) affinity tag, a 42 kDa fusion partner widely used to enhance solubility and simplify purification of recombinant proteins expressed in E. coli. Supplied as 200 µl of working solution, the IgG antibody is validated for western blot detection of MBP-tagged fusion proteins in bacterial lysates, cell lysates, and purified recombinant samples. Typical workflows include confirming expression of MBP fusion constructs, verifying tag removal following protease cleavage, and monitoring yields during amylose affinity chromatography. The polyclonal antibody recognizes multiple epitopes across the MBP tag, providing reliable signal even when the tag is partially denatured during SDS-PAGE. Store at -20 °C in single-use aliquots to preserve activity across repeated freeze-thaw cycles.