Anti-MARK1 Antibody

Produced by Antibodies.com as an unconjugated rabbit polyclonal reagent, this antibody recognizes MAP/microtubule affinity-regulating kinase 1 (MARK1), a serine/threonine kinase that phosphorylates microtubule-associated proteins such as MAP2, MAP4, and tau to control microtubule stability. Supplied as 100 µl of polyclonal antiserum raised in rabbit, the reagent is validated for Western blot and immunohistochemistry on human and mouse samples. It supports studies of neuronal polarity, asymmetric cell division, and tau phosphorylation pathways implicated in Alzheimer's disease research, where MARK1 activity primes tau for subsequent GSK3β-mediated phosphorylation at sites associated with paired helical filament formation. The antibody is also useful for work on mitotic spindle orientation, Wnt signaling, and epithelial polarity, since MARK1 is the mammalian ortholog of the C. elegans Par-1 partitioning kinase. Researchers can apply it to detect endogenous MARK1 in tissue lysates or formalin-fixed paraffin-embedded sections, with the polyclonal format providing recognition of multiple epitopes across the conserved kinase domain. Store refrigerated at 2 to 8 degrees C and avoid repeated freeze-thaw cycles to preserve activity across blots and staining runs.