Anti-MAPKAPK3 Antibody

Stress-activated MAPKAPK3 kinase detection is supported by this rabbit polyclonal IgG reagent from Antibodies.com, supplied as 50 µl of unconjugated liquid stock. The antibody recognises the human, mouse, and rat forms of the kinase, enabling cross-species comparison of p38 MAPK cascade activity in cell culture and tissue specimens. Validated for Western blot and immunofluorescence workflows, the reagent produces a band near the expected molecular weight of the target protein in lysate-based assays and supports cytoplasmic or nuclear staining patterns in fixed cells. MAPKAPK3, also known as MK-3 or 3pK, is phosphorylated and activated by p38-alpha/MAPK14 at residues including Thr-201, Ser-251, and Thr-313, then translocates from the nucleus to the cytoplasm where it phosphorylates substrates such as HSP27/HSPB1, KRT18, KRT20, and TTP/ZFP36. Researchers investigating toll-like receptor signalling in dendritic cells, post-transcriptional regulation of TNF and IL6 mRNAs, or stress responses to growth factors and cytokines use this reagent to monitor endogenous kinase levels. The unconjugated format pairs cleanly with HRP-, Alexa Fluor-, or biotin-conjugated secondary antibodies matched to the chosen detection method.