Anti-MAPKAPK2 (Ab-272) Antibody

MAPK-activated protein kinase 2 (MAPKAPK2, also known as MK2) is a serine/threonine kinase that operates downstream of p38 MAPK and contributes to cellular stress, inflammatory, and cytokine signaling cascades. This rabbit polyclonal reagent from Antibodies.com is raised against a synthetic peptide epitope surrounding residue 272 of human MAPKAPK2, a region that encompasses a key regulatory autophosphorylation site, so it is best suited to studies that probe kinase activation state rather than steady-state expression alone.

The antibody is supplied as an unconjugated preparation in a 100 µl vial volume and has been validated for Western blot and immunohistochemistry workflows on human samples. In WB, it can be paired with an appropriate HRP- or fluorophore-conjugated secondary antibody to detect endogenous MAPKAPK2 from lysates of cytokine- or stress-stimulated cells; in IHC, it supports localization studies in fixed tissue sections, including archival paraffin-embedded material. Host species is rabbit, clonality is polyclonal, and the unconjugated format leaves the choice of label and detection chemistry to the end user.

For handling, keep it at -20 °C in working aliquots to limit freeze-thaw cycles, avoid prolonged exposure to light if a downstream fluorophore is planned, and treat the reagent as research use only. Investigators working on p38-MAPK pathway readouts, cytokine-driven inflammation models, or phospho-regulation of MK2 substrates such as HSP27, tristetraprolin, and eIF4E will find this reagent a focused tool for both routine validation and more mechanistic follow-up experiments.