Anti-MAPK3 Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against a synthetic peptide of human MAPK3 (ERK-1/p44-MAPK) and is designed to detect the endogenous level of total MAPK3 protein in human, mouse, and rat samples. Supplied as an unconjugated antibody at 1.0 mg/mL in a 50 µL aliquot, the product is formulated in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. It is validated for Western blot (WB) and immunohistochemistry (IHC) workflows, making it well suited for signal-transduction and kinase-pathway studies across a broad range of tissues and cell lines. The immunogen corresponds to a conserved region of MAPK3, and the antibody was purified by affinity chromatography against the immunogen to reduce non-specific background. For routine handling, the vial is shipped at 4°C and should be aliquoted upon arrival and stored at −20°C, with repeated freeze/thaw cycles avoided to preserve activity; the 0.02% sodium azide content also permits short-term storage at 4°C for working dilutions. As an RUO (research use only) reagent, this Antibodies.com offering provides a reliable polyclonal tool for laboratories investigating the MAPK/ERK cascade, where MAPK3 (ERK1) partners with MAPK1 (ERK2) to mediate mitogenic and stress responses.