Anti-MAPK14 Antibody

Raised in rabbit against a synthetic peptide derived from human MAPK14, this polyclonal IgG reagent from Antibodies.com binds the ~41 kDa p38 alpha MAP kinase implicated in stress-activated inflammatory signaling, cytokine response pathways, and apoptotic cascades. Affinity-purified and supplied unconjugated in 100 µL of phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the antibody detects endogenous total MAPK14 protein across human, mouse, and rat samples and is validated for western blot, immunohistochemistry, immunoprecipitation, and flow cytometry workflows. Recommended starting dilutions are 1:500-1:2000 for western blot and 1:50-1:200 for immunohistochemistry. The polyclonal format offers broad epitope coverage suitable for probing both basal expression and stress-induced Thr180/Tyr182 dual phosphorylation events triggered by UV exposure, osmotic shock, and inflammatory ligands. Investigators working on signal transduction, cell cycle regulation, and innate immune activation use this reagent to map p38 pathway activity in primary cells and established lines. The vial is shipped at 4°C; aliquot upon receipt and store at -20°C, avoiding repeated freeze/thaw cycles to preserve binding activity. For research use only.