Anti-MAP4K5 Antibody

The MAP4K5 kinase sits near the top of stress-activated MAPK cascades, where it phosphorylates downstream intermediates such as MAP2K4 and helps propagate signals linked to inflammatory cytokine output, JNK activation, and cellular stress responses. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com targets an internal epitope of human MAP4K5 and is validated for western blot detection across human, mouse, and rat samples, with band resolution near the 95 kDa marker expected on standard SDS-PAGE.

MAP4K5, also known as KHS (Kinase Homologous to SPS1/STE20), belongs to the STE20 family of serine/threonine kinases and has been described in published studies as a contributor to TNF-α signaling, JNK pathway activation, and metabolic regulation in liver and adipose tissue. Because the antibody is polyclonal, it recognizes multiple epitopes along the target, which gives a broader detection window than a monoclonal reagent when working with orthologs across species or when endogenous expression is low. Affinity-purified antiserum is supplied at 200 µl per vial in unconjugated format, leaving reporter chemistry and detection conditions fully under the user's control.

Each vial ships with enough volume for routine western blot work over multiple experiments. Store the vial refrigerated upon arrival; aliquoting into single-use volumes preserves activity across experiments and limits freeze-thaw damage to the polyclonal pool. For longer-term storage, freeze aliquots and avoid repeated freeze-thaw cycles. Researchers using the reagent should titrate against their own lysate or tissue preparations to determine the optimal antibody concentration for their specific workflow.