Anti-MAP3K7IP1 Antibody

The polyclonal reagent from Antibodies.com specifically recognizes MAP3K7IP1, also known as TAB1 (TGF-β-activated kinase 1-binding protein 1). MAP3K7IP1 functions as a scaffold that couples TAK1 (MAP3K7) to upstream activators such as TRAF2, TRAF6, and several pattern-recognition receptors, driving downstream activation of NF-κB and JNK/AP-1 in response to pro-inflammatory cytokines, Toll-like receptor engagement, and various cellular stress stimuli. The protein is implicated in innate immunity, inflammatory signaling, TGF-β responses, and stress-activated kinase cascades, and has been studied in the context of cardiac hypertrophy and tissue remodeling.

Raised in Rabbit and supplied as 100 µL of unconjugated polyclonal antiserum, the antibody is validated for Western Blot and Immunohistochemistry applications on Human, Mouse, and Rat samples. The polyclonal format offers recognition of multiple epitopes on the target, which can be advantageous for detecting full-length MAP3K7IP1 as well as its proteolytic fragments or post-translationally modified forms. The unconjugated format leaves the choice of secondary label and detection chemistry to the researcher, supporting both chromogenic and fluorescence-based protocols.

For immunoblotting, working concentrations are typically in the low microgram-per-milliliter range, while IHC signal generally benefits from antigen retrieval optimization tailored to the specific tissue type. Recommended handling includes brief centrifugation of the vial before opening, aliquoting to limit freeze-thaw cycles, and storage at low temperature to preserve reagent activity across repeated experiments.