Anti-MAP3K5 Antibody

This rabbit polyclonal IgG antibody targets MAP3K5, the 155 kDa serine/threonine kinase also known as ASK1 or MAPKKK5, which sits at the head of stress-activated MAPK signaling cascades driven by oxidative damage, endoplasmic reticulum stress, and pro-inflammatory cytokines such as TNF-α. Raised in rabbit and supplied as an unconjugated IgG preparation, it has been validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows against human, mouse, and rat samples, supporting studies of apoptosis regulation, JNK and p38 pathway activation, and cellular responses to reactive oxygen species in both cell culture models and tissue sections.

MAP3K5 is widely studied as a relay point for oxidative and inflammatory stress into downstream JNK and p38 activation, and altered ASK1 activity has been reported in settings ranging from cardiac hypertrophy and neurodegeneration to metabolic disease and tumor biology. Researchers adopting this reagent from Antibodies.com titrate it to working dilutions appropriate for the chosen detection method, with the unconjugated format allowing direct pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents of the user's choice. Each vial contains 50 µL of antibody, and the polyclonal mixture recognizes multiple epitopes on the target, which can improve detection when samples carry post-translational modifications or fixation-induced masking of a single epitope.