Anti-MAP2K6 Antibody

Raised in rabbit against the MAP2K6 dual-specificity kinase, this polyclonal reagent from Antibodies.com targets a key upstream activator of p38 MAPK that drives cellular responses to inflammatory cytokines and environmental stress. The unconjugated IgG is supplied as a 100 µl aliquot and is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples.

MAP2K6 (also known as MKK6 or MEK6) sits at the apex of the stress-activated kinase cascade, phosphorylating p38α/β/γ isoforms on the Thr-Gly-Tyr motif to propagate signals involved in cytokine production, cell cycle arrest, and apoptotic priming. Researchers studying TLR signaling, TNFα responses, cardiac hypertrophy models, or tumor microenvironment crosstalk rely on robust MAP2K6 detection to map where pathway activation diverges from the parallel MKK3- or MKK4-driven branches.

For western blot, the expected band falls near the predicted molecular weight of the MAP2K6 polypeptide; for IHC and IF on fixed tissue sections or cultured cells, citrate-buffer antigen retrieval (pH 6.0) and standard fluorophore-conjugated secondary antibodies give clean cytoplasmic staining. Each lot is affinity-purified and shipped refrigerated on wet ice for short domestic transit; for long-term storage keep the vial at -20 °C in small working aliquots to limit freeze-thaw cycles and preserve antibody activity.