Anti-MAP2K4 (phospho T261) Antibody - Identical to Abcam (ab194820)

Supplied as a 50 µl liquid aliquot, this rabbit polyclonal IgG antibody from Antibodies.com is a phospho-specific reagent raised against MAP2K4 (also called MKK4 or JNKK1) phosphorylated at threonine 261. It is sequence-identical to the Abcam reference reagent ab194820 and is validated for immunohistochemistry (IHC) and immunofluorescence (IF) on human, mouse, and rat specimens, including formalin-fixed paraffin-embedded tissue and fixed cultured cells.

MAP2K4 is a dual-specificity kinase in the stress-activated SAPK/JNK signaling cascade, with threonine 261 located within its activation loop as a key regulatory phosphorylation site. Detecting the phosphorylated form rather than total protein allows researchers to read out the activation state of MAP2K4 in situ, complementing downstream markers such as phospho-c-Jun and phospho-p38 in studies of apoptosis, inflammatory signaling, and cellular stress responses. The unconjugated polyclonal is intended for chromogenic or fluorescent detection in fixed samples; aliquot upon arrival, store refrigerated, and avoid repeated freeze-thaw cycles to preserve phospho-epitope recognition.