Anti-MAP2K3 (phospho S189) Antibody - Identical to Abcam (ab131283) and Novus (NB100-82048)

The Antibodies.com Anti-MAP2K3 (phospho S189) polyclonal antibody is a rabbit-derived primary reagent that selectively detects the activated form of MAP2K3 (also known as MKK3 or MEK3), a dual-specificity kinase acting upstream of p38 MAPK in cellular stress and inflammatory signaling cascades. Phosphorylation at serine 189 within the activation loop is a hallmark of MAP2K3 catalytic activation, making this antibody a useful probe for monitoring kinase pathway engagement triggered by osmotic stress, pro-inflammatory cytokines, or pathogen-associated stimuli.

Supplied as a 100 µl unconjugated IgG preparation, the antibody is validated for Western blot, immunohistochemistry, and immunofluorescence workflows in human and rat samples. As a polyclonal reagent, it binds multiple epitopes around the phospho-S189 region, providing stronger signal intensity and greater tolerance to minor conformational variation than a typical monoclonal reagent. The unconjugated format leaves labeling flexible, allowing researchers to pair it with their preferred secondary antibody or to apply custom fluorophore, biotin, or HRP conjugation strategies tailored to a given assay.

The reagent supports studies of MAPK signaling dynamics, stress-responsive kinase activation, and downstream inflammatory gene regulation in fixed tissues and cultured cell preparations. Researchers can use it to examine the spatial distribution of activated MAP2K3 across tissue sections, track temporal activation kinetics in stimulated cell lysates by Western blot, or visualize subcellular localization by immunofluorescence. Its cross-reactivity across human and rat models enables translational comparisons between cell line experiments, primary tissue specimens, and animal model systems, particularly in research focused on innate immunity, cytokine signaling, and the cellular response to environmental challenge.