Anti-MAP2K2 (phospho T394) Antibody

From Antibodies.com, this rabbit polyclonal IgG antibody recognises MAP2K2 (also known as MEK2) when phosphorylated at threonine 394. It is supplied unconjugated and is validated for Western blot, immunohistochemistry and immunofluorescence applications. The Antibodies.com product listing reports reactivity with human, mouse and rat samples, while the manufacturer datasheet specifies reactivity with human samples. The immunogen is a phospho-specific peptide corresponding to residues surrounding T394 of human MEK2, and the target protein has a reported molecular weight of 44 kDa.

The antibody is provided as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide, and is purified by affinity chromatography. Recommended working dilutions are 1:500–1:2,000 for Western blot, 1:50–1:100 for immunohistochemistry and 1:100–1:200 for immunocytochemistry/immunofluorescence. It is shipped at 4 °C and should be aliquoted and stored at −20 °C upon delivery, with freeze/thaw cycles avoided. The product is accompanied by 3 scientific validation images.