Anti-MAP2K2 Antibody

This polyclonal antibody from Antibodies.com targets MAP2K2 (also known as MEK2), a dual-specificity threonine/tyrosine kinase that phosphorylates MAPK1/ERK2 and MAPK3/ERK1 within the canonical RAS-RAF-MEK-ERK signaling cascade. The reagent is raised in rabbit and purified as an IgG preparation supplied unconjugated in 200 µl volume, validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat samples.

MAP2K2 functions upstream of ERK1/2 to relay mitogenic signals from activated RAF kinases, and germline gain-of-function mutations in the encoding gene are linked to cardiofaciocutaneous syndrome and to several sporadic solid tumors, making reliable detection of the endogenous protein valuable for pathway studies and disease model characterization. Researchers commonly apply this reagent to confirm expression changes after pharmacologic MEK inhibition, to validate knockout or knockdown efficiency in cultured cell lines, and to localize MEK2 in tissue sections from neurological, oncological, and developmental models.

The unconjugated format allows flexible pairing with secondary reagents of the user's choice (HRP, fluorophore, or biotin conjugates) for chromogenic or fluorescence readouts. The polyclonal nature of the host response enables recognition of multiple epitopes on full-length MEK2, which can be advantageous when studying the protein alongside its cleaved or post-translationally modified forms. For handling, aliquot the vial upon receipt to limit freeze-thaw cycles and preserve binding activity over the working lifespan of the antibody.