Anti-MAP2K2 Antibody

Raised in rabbit against the MAP2K2 dual-specificity protein kinase, this polyclonal IgG reagent from Antibodies.com targets the MEK2 enzyme (gene aliases: CFC4, MAPKK2, MKK2, PRKMK2) that activates ERK1 and ERK2 within the MAPK/ERK signaling cascade. The unconjugated antibody is supplied as a 100 µl vial and is validated for western blot and immunohistochemistry on human, mouse, and rat samples, producing a band of approximately 44 kDa on immunoblots of whole-cell lysates. Researchers apply it to study Ras/Raf/MEK-driven proliferation in tumor cell lines, O-GlcNAcylation-linked breast cancer cell migration, and developmental or cardio-facio-cutaneous syndrome models where MAP2K2 germline mutations alter downstream ERK activity. Affinity-purified polyclonal IgG recognizes multiple epitopes on the MAP2K2 protein, supporting detection of the endogenous kinase across human, mouse, and rat tissue sections. For western blot, working dilutions commonly fall in the 1:500–1:1000 range with HRP-conjugated secondary antibodies, while IHC on fixed paraffin-embedded tissue is typically paired with EDTA-based antigen retrieval. Each vial is supplied for research use only and should be stored at -20 °C to preserve IgG stability across repeated freeze-thaw cycles.