Anti-MAP1LC3A Antibody

The MAP1LC3A protein, widely known as LC3A, is a central marker of autophagy — the lysosome-dependent degradation pathway that recycles damaged organelles and misfolded protein aggregates. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against MAP1LC3A and validated for western blot, immunohistochemistry, and immunofluorescence work on human, mouse, and rat samples. Researchers routinely use it to track the conversion of cytosolic LC3A-I into the lipidated, autophagosome-membrane-bound LC3A-II form, a standard readout of autophagic flux in models of neurodegeneration, tumor cell stress responses, and metabolic disease. The reagent ships as 100 µl of a 1 mg/ml solution in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant, and remains stable through repeated freeze-thaw cycles when stored at -20°C. The polyclonal format broadens epitope coverage across the MAP1LC3A target, while the unconjugated presentation gives labs flexibility to pair it with their preferred secondary reagents — HRP, fluorophores, or biotin-streptavidin — for chemiluminescent, fluorescence, or chromogenic detection. Supplied as a research-use-only reagent, the antibody is suitable for both whole-cell lysate analysis and fixed-tissue imaging of endogenous autophagosome formation.