Anti-MAGOH Antibody

The MAGOH protein (Mago Nashi Homolog) is a core component of the exon junction complex (EJC), the multi-protein assembly deposited upstream of exon-exon junctions on newly spliced mRNAs that governs nuclear export, nonsense-mediated mRNA decay, transcript localization, and translation efficiency. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com was raised against the MAGOH target and validated for Western blot and immunohistochemistry on human tissue and cell samples, making it useful for research in RNA splicing regulation, neural development (MAGOH haploinsufficiency and 3p25 copy-number changes have been reported in association with intellectual disability and craniofacial anomalies), and cancer biology where altered EJC activity has been described.

Supplied as 200 µl of liquid antiserum, the antibody is provided without an enzyme or fluorophore attached, leaving the end user free to pair it with HRP, biotin, or fluorescent secondary reagents appropriate to the chosen detection platform. The polyclonal format increases the likelihood of recognizing multiple epitopes on MAGOH, which is helpful when probing native protein complexes or partially degraded material in lysate preparations and fixed tissue sections. Working dilutions should be optimized empirically for each application, starting from standard immunoblotting and immunohistochemistry reference ranges.