Anti-MAD2L1 Antibody

This rabbit polyclonal reagent from Antibodies.com targets MAD2L1 (Mitotic Arrest Deficient 2 Like 1), a key component of the mitotic spindle assembly checkpoint that monitors kinetochore-microtubule attachment and delays the onset of anaphase until every chromosome has achieved proper bipolar orientation on the metaphase plate. Produced in rabbit and supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, this unconjugated antibody is validated for Western blot detection in Human, Mouse, and Rat samples. The unconjugated format leaves labelling chemistry open for downstream HRP, fluorophore, or biotin coupling, while the 100 µl vial supports multiple membranes or longitudinal replicate experiments. For routine handling, aliquot on receipt and store frozen at -20 °C, limiting freeze-thaw cycles to preserve binding activity over time. Researchers in cell cycle biology, checkpoint signalling, and chromosomal instability use anti-MAD2L1 to investigate CDC20 binding dynamics, inhibition of the anaphase-promoting complex (APC/C), and mitotic fidelity in tumour-derived and primary cell models.