Anti-MAD2L1 Antibody

MAD2L1 is a core component of the mitotic spindle assembly checkpoint, the surveillance mechanism that delays anaphase onset until every kinetochore has attached to spindle microtubules. This unconjugated, rabbit polyclonal IgG from Antibodies.com is affinity-purified and supplied at a 50 µl volume as a primary antibody for research use only. Validated for Western blot (WB) and immunofluorescence (IF), it recognizes the endogenous ~24 kDa MAD2 protein in both human and mouse samples, supporting studies of chromosome segregation fidelity, mitotic checkpoint complex (MCC) assembly with CDC20 and BUBR1, and the cellular response to antimitotic agents such as taxanes and vinca alkaloids. Researchers deploy the reagent to probe spindle assembly checkpoint (SAC) activity in tumor cell lines exhibiting aneuploidy or chromosomal instability, to track MAD2 localization at unattached kinetochores during prometaphase, and to correlate MAD2 expression with tumor outcomes in T-ALL and hepatocellular carcinoma models where conditional SAC disruption drives whole-chromosome gain and loss. The unconjugated format allows the antibody to be paired with secondary reagents for chemiluminescent western blot imaging or for indirect immunofluorescence detection of kinetochore-bound MAD2, and the 50 µl vial size suits laboratories running moderate-throughput SAC profiling or comparing MAD2 levels across multiple cancer cell lines.