Anti-MAD1L1 Antibody

Produced in rabbit against the Mitotic Arrest Deficient 1 Like 1 protein, this polyclonal antibody from Antibodies.com recognizes MAD1L1, a core component of the mitotic spindle checkpoint that monitors kinetochore attachment and delays anaphase onset until every chromosome is correctly bi-oriented on the spindle. MAD1L1 partners with MAD2L1 at unattached kinetochores to generate the mitotic checkpoint complex, and its dysregulation is a frequent topic of cancer research, including studies of lung cancer, prostate cancer, and lymphoid malignancies where checkpoint weakening contributes to aneuploidy. The unconjugated reagent is supplied as 100 µl and is validated for western blot, immunohistochemistry, and immunofluorescence in human, mouse, and rat samples. Cell cycle and DNA damage response laboratories use the antibody to track kinetochore localization, monitor checkpoint signaling after spindle disruption with nocodazole or MPS1 inhibitors, and probe how post-translational modifications influence MAD1L1 turnover. The polyclonal format detects multiple epitopes, supporting consistent staining of full-length protein and modified species across fixed tissue sections and cell preparations, with reproducible nuclear and kinetochore-associated IF signal. Aliquot on receipt and store at -20 °C; the reagent tolerates standard short-term handling, and limiting freeze-thaw cycles preserves affinity across long projects. Researchers studying spindle assembly checkpoint fidelity, mitotic exit, chromosomal instability, and tumor suppressor pathways will find this reagent useful for both hypothesis-driven experiments and validation screens.