Anti-MAD1L1 Antibody

Produced in rabbit and validated for Western blot, immunohistochemistry, and immunofluorescence, this polyclonal reagent from Antibodies.com targets the Mitotic Arrest Deficient 1 Like 1 (MAD1L1) protein, a core component of the spindle assembly checkpoint that monitors kinetochore-microtubule attachment during mitosis. The antibody is supplied as 100 µl of unconjugated protein at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and cross-reacts with Human, Mouse, and Rat samples, supporting cell cycle, aneuploidy, and chromosomal instability studies across standard model systems. Researchers use it to profile endogenous MAD1L1 expression by Western blot, localize the protein in fixed tissue sections by immunohistochemistry, and visualize kinetochore-associated MAD1L1 by immunofluorescence in dividing cells. Because MAD1L1 partners with MAD2 at unattached kinetochores to delay anaphase onset, this reagent is well suited to investigations of mitotic checkpoint fidelity, the consequences of MAD1L1 dysregulation in tumor cell lines, and the role of the MAD1–MAD2 complex in chromosome mis-segregation. For best results, store refrigerated and limit freeze-thaw cycles to preserve reagent activity.