Anti-M-CSF Antibody

The rabbit polyclonal antibody recognizes M-CSF (macrophage colony-stimulating factor, also called CSF1), a homodimeric cytokine that drives survival, proliferation, and differentiation of cells along the monocyte and macrophage lineage. Raised against the human immunogen, the reagent is provided as 100 µL of unconjugated antiserum from Antibodies.com and is validated for Western blot and ELISA applications across human research samples.

M-CSF signaling is a focal point in studies of osteoclastogenesis, inflammatory cytokine cascades, and the recruitment of tumor-associated macrophages in solid tumor microenvironments. Investigators use this reagent to confirm endogenous ligand expression in conditioned media from cultured primary monocytes, macrophage cell lines, and tissue homogenates, and to verify upregulation following stimulation with pro-inflammatory mediators such as LPS or IL-1β.

The unconjugated format supports flexible downstream secondary labeling with HRP, biotin, or fluorophore-conjugated reporters, making it straightforward to integrate into established immunodetection workflows. For best results, aliquot upon first receipt and store at -20°C to preserve antibody activity across multiple freeze-thaw cycles, then centrifuge the vial briefly before opening to recover the full liquid volume.

Researchers working on macrophage biology, bone remodeling pathways, and cytokine cross-talk in cancer and chronic inflammatory disease models will find this polyclonal reagent from Antibodies.com a useful tool for validating M-CSF expression by an orthogonal antibody clone.