Anti-LIMS1 Antibody

Focal adhesion researchers and labs studying integrin-linked kinase signaling use this rabbit polyclonal reagent from Antibodies.com to detect the LIM and senescent cell antigen-like domain 1 (LIMS1) protein, also called PINCH-1. Supplied as a 100 µl liquid aliquot, the antibody is validated for western blot and immunohistochemistry on human and mouse specimens and arrives unconjugated for maximum flexibility in labeling chemistry.

LIMS1 operates as a focal adhesion scaffold that pairs with integrin-linked kinase and Nck-2 to convey integrin activation into downstream effectors controlling cell migration, spreading, and survival. Published studies have examined its expression in cardiac development, renal fibrosis, tumor invasion, and wound healing, and the protein is often discussed alongside epithelial-mesenchymal transition and metastatic programs. The unconjugated format enables pairing with HRP, biotin, or fluorescent secondaries in detection chemistries optimized in each laboratory.

For western blot work on cell lysates, the antibody combines with standard chemiluminescent or fluorescent detection reagents. For immunohistochemistry on formalin-fixed, paraffin-embedded material, antigen retrieval and blocking conditions should match the fixation protocol of the tissue. Each vial covers multiple optimization runs and routine blot or slide batches without exhausting the stock.