Anti-LIMK2 Antibody

Validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this rabbit polyclonal antibody from Antibodies.com targets LIMK2 (LIM domain kinase 2), a serine/threonine kinase that phosphorylates cofilin to regulate actin filament turnover. The reagent is supplied as 100 µl of unconjugated antibody, suitable for immunostaining and protein analysis workflows in research use only settings.

LIMK2 expression and phosphorylation influence cell motility, neurite outgrowth, cell cycle progression, and tumor cell invasion. The antibody detects LIMK2 in human, mouse, and rat samples, supporting studies of metastatic mechanisms in breast, prostate, and lung cancer, as well as synaptic plasticity and neuronal morphogenesis in nervous system tissue.

As a polyclonal reagent, the antibody recognizes multiple epitopes on LIMK2, which can improve signal reliability across varied experimental conditions. The unconjugated format allows flexible pairing with secondary antibodies for chromogenic, fluorescent, or chemiluminescent detection.

Common applications include analysis of LIMK2 in cell lysates by Western blot, localization in fixed cells and tissue by immunofluorescence, and antigen detection in paraffin sections by immunohistochemistry. Researchers frequently examine LIMK2 alongside related cytoskeletal regulators such as cofilin, TESK, and SSH1 phosphatase in vitro.