Anti-LIMK-1 / 2 Antibody

LIM domain kinases 1 and 2 phosphorylate cofilin, regulating actin filament turnover, dendritic spine morphogenesis, and cell motility downstream of Rho GTPase signaling. This unconjugated rabbit polyclonal antibody, raised against LIMK-1 and LIMK-2, allows researchers to probe both kinase isoforms in cytoskeletal remodeling, neuronal development, and metastatic signaling studies. Supplied by Antibodies.com as a 100 µl liquid aliquot, the reagent is validated for western blot, immunohistochemistry, and ELISA workflows. Reactivity has been confirmed across human, mouse, rat, and monkey samples, supporting cross-species investigations in cell lysates, formalin-fixed tissue sections, and recombinant protein assays. The polyclonal format detects multiple epitopes on each LIMK isoform, which is advantageous when monitoring expression changes, isoform switching, or post-translational modifications during experimental manipulation. Working aliquots are recommended for routine use to limit repeated freeze-thaw cycles, and the unconjugated format offers flexibility to conjugate with HRP, biotin, or fluorophores depending on the chosen detection platform.