Anti-LIMA1 Antibody

Produced by Antibodies.com, this unconjugated rabbit polyclonal IgG antibody specifically targets the LIMA1 protein, a cytoskeletal regulator involved in actin filament bundling and cell junction dynamics. The reagent is supplied at 100 µl and validated for Western blot (WB) applications on human samples, fitting cell biology and cancer research workflows where LIMA1 expression is profiled.

LIMA1, also referred to as EPLIN (LIM domain and actin-binding protein 1), is investigated in the context of epithelial-mesenchymal transition, focal adhesion turnover, and stress fiber organization, where it bridges the actin cytoskeleton with membrane-associated complexes. Researchers apply this reagent to probe endogenous LIMA1 levels in lysates prepared from cultured human cell lines and primary tissues.

For Western blot, the antibody is incubated after SDS-PAGE separation and transfer to nitrocellulose or PVDF membranes, followed by chemiluminescent or HRP-based detection. Recommended handling is to refrigerate at 4 °C for short-term use; for longer-term storage, aliquot the antibody and freeze at -20 °C, avoiding repeated freeze-thaw cycles. For research use only.