Anti-LIG4 Antibody

From Antibodies.com comes a primary antibody reagent: a rabbit polyclonal IgG preparation targeting DNA Ligase IV (LIG4). The unconjugated product is supplied as a liquid and is accompanied on the manufacturer's product page by two scientific images.

The antibody is affinity purified and is formulated in Phosphate Buffered Saline, pH 7.3, containing 50% glycerol and 0.01% thiomersal. The immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 1–210 of human DNA Ligase IV (NP_002303.2). The target protein has a reported molecular weight of 110 kDa, and the antibody concentration is described as lot specific.

Validated applications are WB and IHC, with recommended dilutions of 1:500–1:1,000 for WB and 1:50–1:200 for IHC. The Antibodies.com product listing also lists IF among the applications. Reactivity is given as Human, Mouse and Rat on the manufacturer's datasheet, while the Antibodies.com product page lists Human and Mouse. The product ships at 4 °C and should be aliquoted and stored at −20 °C, with freeze/thaw cycles avoided.