Anti-LIG3 Antibody

Raised in rabbit against DNA Ligase III (LIG3), this unconjugated polyclonal reagent from Antibodies.com supports DNA repair research through Western blot, immunohistochemistry, and immunofluorescence workflows. The antibody recognises an epitope conserved across the human, mouse, and rat orthologues, making it useful for comparative studies of base excision repair and single-strand break rejoining in nuclear and mitochondrial extracts.

The IgG fraction is supplied at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, a glycerol-rich formulation that remains liquid at –20 °C and tolerates repeated freeze-thaw handling better than fully aqueous preparations. Each vial contains 100 µl of ready-to-use working stock, providing ample volume for multiple Western blot, IHC, and IF experiments from a single aliquot. Sodium azide acts as a microbial preservative, an important consideration for downstream users pairing the primary antibody with HRP-conjugated secondaries.

LIG3 partners with XRCC1 to seal single-strand nicks during short-patch base excision repair, and the mitochondrial isoform handles analogous repair inside the organelle. Researchers probing PARP1-dependent repair, XRCC1 scaffold function, or mtDNA maintenance in cancer, neurodegeneration, and ageing models routinely use this reagent to track endogenous protein levels and subcellular localisation. The unconjugated format leaves the choice of detection label, fluorophore, or biotinylation entirely to the end user.