Anti-LIF Antibody

The leukemia inhibitory factor (LIF) is a pleiotropic cytokine belonging to the IL-6 superfamily, and this unconjugated rabbit polyclonal reagent from Antibodies.com binds the human LIF protein for use in Western blot and ELISA workflows. LIF signals through a heterodimeric receptor complex composed of LIFR and the signal transducer gp130, activating the JAK/STAT3, MAPK, and PI3K/AKT cascades that sustain embryonic stem cell self-renewal, drive neuronal and hematopoietic differentiation, and modulate inflammatory responses. Researchers investigating reproductive biology, embryo implantation, trophoblast function, neural stem cell plasticity, and cancer microenvironments rely on detection of endogenous LIF protein to characterize autocrine and paracrine signaling in primary cells and tissue lysates.

Raised in rabbit and supplied as 100 µl of polyclonal antiserum in an unconjugated format, the reagent is compatible with standard immunodetection chemistries, including HRP- or fluorophore-conjugated secondary antibodies, and can be paired with recombinant human LIF as a positive control during Western blot validation. Because LIF expression shifts with cellular context and is frequently dysregulated in solid tumors and inflammatory disease models, this reagent is commonly deployed alongside housekeeping markers such as β-actin or GAPDH to normalize band intensity across treated and untreated samples.

Aliquot the antibody upon receipt and store at -20 °C in single-use portions to limit freeze-thaw cycles and preserve long-term binding activity. Brief handling on ice is recommended when diluting for downstream applications.