Anti-LIF Antibody

The rabbit polyclonal antibody from Antibodies.com is raised against Leukemia Inhibitory Factor (LIF), a pleiotropic cytokine of the IL-6 family that signals through the gp130/JAK/STAT3 axis and supports self-renewal of pluripotent stem cells. Supplied as an unconjugated reagent, this primary antibody is validated for Western blot detection of the LIF protein in human, mouse, and rat samples.

Each vial contains 100 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. The polyclonal format provides recognition of multiple epitopes across the LIF target, helpful when studying cytokine expression patterns in stem cell maintenance, embryonic development, neurogenesis, bone metabolism, and inflammatory signaling research. The unconjugated presentation allows flexible pairing with horseradish peroxidase or fluorophore-conjugated secondary reagents in standard immunoblotting workflows.

For handling, the antibody should be refrigerated; aliquot and freeze at -20°C for longer-term storage, avoiding repeated freeze-thaw cycles. Brief centrifugation before use helps recover full volume from the vial. Researchers investigating LIF-mediated pathways in regenerative biology, cancer stemness, or inflammatory disease models will find this reagent appropriate for Western blot protocols.