Anti-LGR5 Antibody

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) is a seven-transmembrane receptor that binds R-spondin ligands and amplifies Wnt/β-catenin signalling. It marks actively cycling adult stem cells in intestinal crypts, colonic epithelium, gastric glands, and hair follicles, and is also used to identify cancer stem cell populations in colorectal and gastric tumours. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against LGR5 and supports two routine readouts: Western blot detection of the endogenous receptor in lysates prepared from human, mouse, and rat tissue, and flow cytometry labelling of surface LGR5 on dissociated cells for stem-cell enrichment studies. Researchers in gastrointestinal cancer research, intestinal organoid biology, and regenerative medicine use the antibody to track lineage tracing, characterise tumour-initiating subsets, and probe Wnt pathway activity in epithelial homeostasis models. The product is supplied as 100 µL of polyclonal antiserum in a single vial and should be stored refrigerated to preserve activity. Species reactivity has been confirmed in human, mouse, and rat samples by Western blot, making the reagent broadly applicable across common model systems.