Anti-LEKTI (H525) Antibody

Offered through the Antibodies.com catalogue, this polyclonal reagent is raised in rabbit against the lympho-epithelial Kazal-type-related inhibitor (LEKTI), encoded by the SPINK5 gene. It is supplied as a 50 µl liquid at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, and is unconjugated, with IgG as the immunoglobulin class. The antibody was generated against a synthetic peptide corresponding to amino acids 496-543 of human LEKTI and is recommended for Western blotting and immunohistochemistry on human samples. Recommended working dilutions reported for comparable LEKTI (H525) reagents are approximately 1:500-1:1000 for WB and 1:50-1:200 for IHC. For storage, vials should be kept at 4 °C for short-term use and aliquoted and stored at –20 °C for longer term, with repeated freeze-thaw cycles avoided. Each vial of this Antibodies.com primary antibody is validated as a research-grade tool for studies of epithelial differentiation, mucosal barrier function, and Netherton syndrome, the autosomal recessive skin disorder caused by loss-of-function mutations in SPINK5.