Anti-LCK Antibody

This rabbit polyclonal reagent from Antibodies.com targets LCK, a 56 kDa Src-family tyrosine kinase expressed predominantly in T lymphocytes and required for early T-cell receptor signaling. The unconjugated IgG preparation is supplied as a 200 µL vial and is validated for western blot, immunohistochemistry, immunofluorescence, and flow cytometry workflows on human and mouse samples. The antibody detects both the active phosphorylated form and total LCK in Jurkat and primary peripheral blood mononuclear cell lysates, supporting studies of thymocyte development, T-cell activation, and lymphoid malignancies. Researchers commonly apply it to phospho-LCK signaling readouts, western blot analysis of lymphocyte lysates, IHC staining of lymphoid tissue sections, IF imaging of activated immune cell cultures, and intracellular flow cytometry panels. Its polyclonal format provides broader epitope coverage than monoclonal alternatives, increasing recognition of conserved regions across LCK isoforms where sequence identity is high.