Anti-Lamin B1 Antibody

This rabbit polyclonal antibody specifically targets Lamin B1, a type V intermediate filament protein that forms part of the nuclear lamina in eukaryotic cells. Provided by Antibodies.com as a 100µl aliquot of unconjugated antiserum, the reagent is validated for Western blot and ELISA workflows and cross-reacts with Human, Mouse, and Rat samples. The polyclonal format increases the number of epitopes recognized within the target, which is useful when probing denatured lysates or partially degraded nuclear matrix preparations.

Lamin B1 functions as a structural scaffold at the inner nuclear membrane and serves as a marker for nuclear envelope integrity in cell biology and neuroscience research. Loss of Lamin B1 expression has been documented in models of cellular senescence and DNA damage response, while duplications and certain point mutations are linked to autosomal dominant leukodystrophy (ADLD). Because the antibody recognizes an evolutionarily conserved region of the protein, it can be used for normalization in fractionation studies comparing nuclear versus cytoplasmic compartments across multiple model organisms. For storage, refrigerate the vial and divide into single-use aliquots to minimize freeze-thaw cycles of the polyclonal reagent.