Anti-Lamin A/C (S17) Antibody

The polyclonal antibody offered by Antibodies.com targets an epitope surrounding serine 17 within the N-terminal head domain of human Lamin A/C, a region that becomes phosphorylated during mitotic nuclear envelope breakdown. Raised in rabbit and affinity-purified, the reagent is supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, adjusted to approximately pH 7.2. Each vial delivers 50 µl of antibody suitable for probing both the 74 kDa Lamin A and 65 kDa Lamin C isoforms in cell lysates and tissue sections.

Validated applications include Western blot and immunohistochemistry on samples originating from human, mouse, and rat sources. In Western blot, the antibody detects the characteristic doublet of Lamin A and Lamin C, while in immunohistochemistry it labels the nuclear rim of intact cells. Recommended storage is refrigerated at 4 °C; protect from light and avoid repeated freeze-thaw cycles by working from small aliquots to preserve binding activity. Sodium azide is included as a preservative. The host species is rabbit, and the clonality is polyclonal, with reactivity confirmed across human, mouse, and rat. For research use only, this unconjugated primary antibody integrates cleanly with secondary detection reagents of the user's choice.