Anti-Ku70 / 80 Antibody

This polyclonal primary antibody from Antibodies.com targets the Ku70/Ku80 heterodimer, a nuclear protein complex essential for non-homologous end joining DNA double-strand break repair and telomere maintenance. Raised in rabbit and supplied as an unconjugated reagent in a 100 µl vial, it is validated for western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

The Ku heterodimer binds broken DNA ends and recruits the DNA-PK catalytic subunit, making reliable detection valuable for studies of genomic instability, DNA damage response signaling, and chromatin remodeling. Researchers investigating V(D)J recombination, radiation-induced damage, or age-related telomere attrition use this reagent to track endogenous expression levels, localize Ku70/Ku80 within nuclear compartments, and compare isoform abundance between treated and control mammalian tissues. As a polyclonal reagent, it recognizes multiple epitopes on the target, which can improve signal strength in tissues where antigen accessibility varies.

For western blot, the antibody is applied to whole-cell or nuclear lysates under reducing conditions; for immunohistochemistry, antigen retrieval with citrate buffer is commonly used on formalin-fixed, paraffin-embedded sections; and for immunofluorescence, it is paired with fluorophore-conjugated secondary antibodies on cultured cells or cryosections. The vial should be kept refrigerated upon arrival; for longer-term storage, aliquoting and freezing the preparation is recommended to limit freeze-thaw cycles. Brief centrifugation before use recovers full volume from the container.

This reagent is intended for research use only and is not validated for diagnostic or therapeutic procedures.