Anti-KRT23 Antibody

Raised in rabbit against a recombinant human KRT23 immunogen and affinity purified, this unconjugated polyclonal IgG reagent from Antibodies.com targets the type I intermediate filament protein keratin 23. KRT23 contributes to epithelial structural integrity and is studied in the context of differentiation and histone-deacetylase-inducible gene regulation; its 17q12-q21 chromosomal locus and aliases CK23 and HAIK1 are widely cited in cytokeratin literature.

Supplied as 200 µl of liquid stock at standard IgG concentration, the antibody is validated for western blot and immunohistochemistry workflows. Recommended starting dilutions are 1:500-1:2000 for western blot on denatured lysates resolved by SDS-PAGE, and 1:50-1:100 for IHC on formalin-fixed paraffin-embedded tissue. In western blot, an observed band is reported at approximately 48 kDa, consistent with the calculated mass of full-length keratin 23 in human epithelial samples.

Reactivity has been confirmed against human and mouse samples, supporting comparative epithelial studies across species. The unconjugated format lets researchers pair the reagent with their own detection chemistry - chromogenic DAB, fluorescent labels, or HRP-conjugated secondaries - rather than a fixed reporter. For research use only. Store the vial refrigerated at 4°C, and for routine use, divide into single-use working aliquots to limit freeze-thaw cycling and preserve epitope recognition across multi-week experiments.