Anti-KLK5 Antibody

Raised in rabbit against a synthetic peptide corresponding to residues near the C-terminal of human kallikrein-related peptidase 5, this polyclonal antibody from Antibodies.com detects endogenous levels of total KLK5 protein in human and mouse samples. The immunogen design targets a conserved region of the trypsin-like serine protease encoded by the KLK5 gene, supporting studies of stratum corneum desquamation, hair follicle cycling, and KLK5 dysregulation in ovarian, breast, prostate, and pancreatic tumor tissue.

Supplied as 100 µl of unconjugated antiserum at 3.2 mg/mL in PBS pH 7.3 with 0.05% sodium azide and 50% glycerol, the reagent is antigen affinity purified and validated for Western blot at a 1/1600 working dilution on 40 µg mouse brain lysate per lane, with chemiluminescent detection. For immunohistochemistry, the antibody has been validated on paraffin-embedded human ovarian cancer sections at a 1/100 dilution, where it localizes KLK5 expression in keratinocyte layers and tumor tissue. Image data on the supplier site is filed under catalogue reference 36941.

Storage guidance from Antibodies.com: shipped at 4°C for stability in transit, then aliquoted and stored at -20°C for long-term use, with repeated freeze-thaw cycles avoided to preserve activity. Researchers investigating protease regulation, inflammatory dermatoses, or tumor microenvironment remodeling can pair this reagent with HRP- or fluorophore-conjugated secondary antibodies; small aliquots are recommended when preparing diluted working stocks. For research use only.