Anti-KLK1 Antibody

From Antibodies.com, this Rabbit polyclonal IgG antibody recognises KLK1 (Tissue kallikrein) and is supplied unconjugated. The product is validated for Western Blotting (WB), and additional manufacturer documentation indicates validation for Immunohistochemistry (IHC) as well, with demonstrated reactivity against Human, Mouse, and Rat samples. The antibody detects endogenous levels of total KLK1 protein.

Manufacturer descriptions cite differing immunogens across product variants, including a recombinant fusion protein corresponding to amino acids 25–262 of human KLK1, a recombinant protein of human KLK1, and recombinant full-length human KLK1. Reported molecular weight values include 22 kDa and approximately 28 kDa. Formulation is variously described as a liquid at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol; or in PBS pH 7.3 with 50% glycerol and 0.02% sodium azide; or in PBS with 0.1% sodium azide and 50% glycerol.

The antibody is purified by affinity chromatography, with one source reporting > 95% purity by SDS-PAGE. Recommended dilutions are 1:500–1:2,000 for WB and 1:100–1:500 for IHC. The product is shipped at 4°C and should be aliquoted and stored at –20°C, avoiding freeze/thaw cycles.