Anti-KLF4 Antibody

KLF4 (Krüppel-like factor 4) is a zinc-finger DNA-binding transcription factor studied for its roles in epithelial differentiation, intestinal homeostasis, cell-cycle checkpoint control, and somatic cell reprogramming, where it functions alongside OCT4, SOX2, and c-MYC as one of the four original Yamanaka factors used to generate induced pluripotent stem cells from differentiated somatic lineages.

This polyclonal reagent from Antibodies.com is raised in rabbit, affinity-purified, and supplied unconjugated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant. The product ships as a 100 µl vial and is validated for Western blot and immunohistochemistry workflows on human, mouse, and rat specimens, making it broadly applicable across colon, skin, and neural tissue studies.

For best results, store the vial at -20 °C in small working aliquots to limit freeze-thaw cycles and preserve binding activity over the working shelf life of the antibody. Polyclonal recognition of multiple epitopes on the KLF4 protein typically yields stronger signal in formalin-fixed paraffin-embedded tissue sections and provides broader detection than monoclonal reagents raised against the same target.

Common research applications include verification of stemness markers during reprogramming experiments, characterisation of tumour-suppressor activity in colorectal and gastric carcinoma models, and analysis of gut-enriched KLF4 expression in inflammatory bowel disease and skin barrier studies.