Anti-KLF3 Antibody

KLF3, also called BKLF, is a CtBP-interacting transcriptional repressor within the Krüppel-like zinc-finger family, implicated in erythropoiesis, B-cell maturation, adipogenesis, and neural development. This unconjugated rabbit polyclonal reagent from Antibodies.com recognizes endogenous KLF3 protein and has been validated for western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

The product is supplied as a 100 µl volume at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol. Because the antibody is unconjugated, researchers retain full flexibility to pair it with HRP-, biotin-, Alexa Fluor-, or Dylight-conjugated secondaries depending on the assay's detection chemistry. Polyclonal recognition provides broad epitope coverage, useful when probing multiple splice isoforms or working across species with conserved sequence regions.

Storage recommendation: store the vial at -20 °C in single-use aliquots to avoid repeated freeze-thaw cycles, and protect it from prolonged light. Typical western blot loading uses 1–2 µg/ml primary antibody in 5% non-fat milk TBST, while IHC on paraformaldehyde-fixed paraffin sections and IF on paraformaldehyde-fixed cultured cells commonly use empirically titrated dilutions. KLF3 expression is reported in hematopoietic lineages including erythroid progenitors and B-lymphoid cells, in adipose tissue, and in several regions of the developing central nervous system, supporting studies of hematopoietic differentiation, metabolic regulation, and developmental gene-expression programs.