Anti-Ki-67 Antibody

A recombinant rabbit monoclonal reagent targeting the Ki-67 nuclear proliferation antigen enables laboratories to identify actively dividing cells in tissue sections, cultured cells, and protein extracts. The Ki-67 protein, encoded by the MKI67 gene, is expressed during G1, S, G2, and M phases of the cell cycle but is absent in quiescent G0 cells, making it a well-established marker of cellular proliferative activity in tumor biology and cell cycle research.

This unconjugated antibody from Antibodies.com is supplied as 100 µl of ready-to-use reagent in a stabilizing buffer of TBS pH 7.4 with 1% BSA, 40% glycerol, and 0.05% sodium azide as a preservative. The recombinant production format in a rabbit host supports batch-to-batch consistency for sensitive immunodetection workflows.

Validated applications include Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Flow Cytometry, allowing a single reagent to be used across protein-level, tissue-level, and single-cell analyses. Reactivity has been confirmed against human samples, supporting studies of tumor biopsies, breast carcinoma grading, neuroendocrine tumor classification, hematopoietic progenitor characterization, and cell cycle regulation research.

The included glycerol cryoprotectant helps maintain antibody activity through routine bench-top handling, while aliquoting is recommended to limit repeated freeze-thaw exposure when the reagent is used across multiple experiments.